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However, it was controversial whether mature erythrocytes, terminally differentiated organelle-lacking cells, can undergo RCD

Cell pellets were resuspended in LDOX lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 0.01 mg ml 1 DNAseI) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator

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The pathological processes involved are profound and complex, and can be systematically characterized by three major aspects: structural disruption, functional dysregulation, and temporospatial dynamics with significant clinical implications