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Immunohistochemical analysis confirmed that OSR downregulated inflammatory mediators and oxidative stress markers while upregulating GPX4 and SLC7A11 expression in joint tissues.Furthermore, OSR demonstrated excellent systemic tolerability, with consistent body weight and preserved histoarchitecture across major organs, indicating minimal offtarget toxicity 32,33 .Current pharmacological treatments for OA primarily provide symptomatic relief rather than disease modification.While NSAIDs and analgesics provide transient pain relief, they are ineffective in rebuilding cartilage or interrupting the chronic inflammatory and oxidative feedback loops that propel osteoarthritic progression 34

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The gene for MSA-DH was cloned into MCS1 of pACYCDuet-1 via NotI and NcoI restriction sites and the gene for MDH was cloned into MCS2 via NdeI and XhoI restriction sites yielding pACYCDuet-1- msadh - mdh