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Cells were seeded in 6-well plates until they reached 80% confluence for transfection of siRNA with Lipofectamine 2000 reagent according to the manufacturer's protocols

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AOD9604 AOD9604 is a synthetic peptide analog derived from the C-terminal region (176191) of human growth hormone (HGH), consisting of a defined amino acid sequence produced through controlled peptide synthesis

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The disruption of protein synthesis results in the production of non-functional or toxic peptides, leading to bacterial cell death ( Amikacin exerts bactericidal effects against both Gram-positive and Gram-negative bacteria, including strains resistant to other aminoglycosides like gentamicin and tobramycin