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Blots were incubated overnight at 4 C with HRP-conjugated anti-nitrotyrosine mouse mAb (1:2500 dilution in CanGetSignal solution 1,NKB-101, Toyobo, Osaka, Japan), followed by ECL-detection on a FUSION SOLO.7S.EDGE imaging system (Vilber Lourmat, Marne-la-Valle, France)

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Acknowledgments We would like to thank Clara Marin Briggiler for her insightful comments, and Nria Correa Maas for her contribution with statistical analyses

They employed a subnanomolar peptide, TRAP binding peptide (TBP) that has a high affinity for TRAP