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Histology and immunohistochemistry Fresh mouse kidneys were fixed in 10% neutral buffered formalin for 24 h, washed once with PBS, then stored in 70% ethanol at 4 C until further processing

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GLP-1 drugs are generally contraindicated Personal or family history of medullary thyroid cancer (MTC): GLP-1 drugs carry a boxed warning for thyroid C-cell tumors in rodent studies Multiple Endocrine Neoplasia syndrome type 2 (MEN 2): Increased MTC risk History of pancreatitis: GLP-1 drugs may increase pancreatitis risk Type 1 diabetes: Not studied

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