Folate, vitamin B6, and vitamin B12 deficiencies are due to poor dietary intakes, illness, genetic defects that result in impaired absorption or transport, or a combination of these factors.9 Although clinical deficiencies of these vitamins are uncommon in the United States and are usually limited to individuals with genetic mutations, low or marginal deficiencies have been reported, particularly among nonwhite and elderly populations, and women of childbearing age
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To visualize cell nuclei, we applied 4,6-diamidino-2-phenylindole (DAPI) staining (Santa Cruz, SC3598), which allowed for clear identification of nuclear localization within the tissue sections
[28]Current Patent Assignee: NITROMED - US5932538, 1999, A