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All can be consequential, but some have more weight than others

These seem like minor details until you realize you are injecting degraded or inactive product and wondering why nothing is happening

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As soon as the supernatant was centrifuged, 400 mL of the supernatant was poured into the corresponding EP tube and stored overnight at 20 C in the refrigerator before being centrifuged at 12,000 r/min for 3 min at 4 C, and approximately 2000 mL of supernatant was collected in the injection bottle liner for on-board analysis

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