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The peroxidation of the PUFA chains leads to the destruction of the phospholipid bilayer membrane structure of the cell enhancing membrane permeability, which ultimately leads to cell death [58, 59]

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Notably, the hepsin / mice exhibited a more pronounced increase in ROS levels after thioacetamide treatment compared with wild-type mice, which aligned with our hypothesis that hepsin / mice experience heightened ROS stress in the early stages of APAP exposure due to their elevated expression of GJ proteins (Fig