2.2 In vitro Cell culture Human neuroblastoma SH-SY5Y cells were cultured in Dulbeccos Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum and 1% penicillin-streptomycin
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2.3 Manifestations of nervous system injury METH abuse induces neurological damage that manifests as cognitive impairment, psychiatric symptoms, and motor dysfunction, with clear distinctions between the acute and chronic phases of use