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We screened candidate mutant lines by PCR and Sanger sequencing and identified the Atg8a 12 allele that carries an 8-bp deletion based on sequencing data (Fig S2A), which causes frameshift in all isoforms of the Atg8a gene

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This work was supported by R35HL139726 (ESN), R01HL156670 (CCS), K24HL150630 (CCS), and 5R01HL157973 (SF)