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Briefly, 10 L of CCK-8 reagent (Biosharp, Hefei, China) was added to each well and then the 96-well plate was continuously incubated at 37 C for 1 h

This metabolic transition is considered adaptive in regenerative contexts

To address these limitations, there is an urgent need to establish consensus-driven, multiomics frameworks, including integrated metabolomic, proteomic, and transcriptomic profiling, to objectively evaluate aging trajectories and therapeutic interventions

Applying it to specific situations is another

IDO, an enzyme expressed by tumor cells, catalyzes the conversion of tryptophan into kynurenine, leading to a local depletion of tryptophan in the tumor microenvironment [53]