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Five percent of fragmented RNA was preserved as input, while the remaining RNA underwent m6A-immunoprecipitation (m 6 A-IP) using the EpiMark N6-Methyladenosine Enrichment Kit.For m 6 A-IP, 1 g of m6A-specific antibody or 1 g of IgG (Pierce) was added to 40 L of Protein G Magnetic Beads and incubated with orbital rotation for 1 h with gentle rotation at RT

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After 96 h of culture in a low-glucose medium, isolated PSCs expressed desmin, vimentin, and GFAP (Fig

Yiming Yin, S.-C