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However, the double mutant Asn240Thr/Leu244Arg clearly shows that Thr as the 1 st activity controller in combination with Arg as the 2 nd activity controller converts the TYR from walnut ( jr PPO1) into a CO as it did no longer accept tyrosine and tyramine

Reactions were stopped 16 hours later by addition of formic acid to a final concentration of 0.1%

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Together, these three compounds address complementary mechanisms that single-peptide protocols may not fully engage