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Randomized Controlled Trials (RCTs): Some studies [e.g., (6, 810)] applied strict designs (e.g., double blind, placebo-controlled trials) which tend to reduce selection bias and performance bias

Small pilot studies in women with PCOS have suggested potential benefits on insulin sensitivity and hormonal parameters, but robust, large-scale trials examining weight loss outcomes specifically are lacking

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Immunohistochemistry For immunohistochemistry, paraffin sections of liver and intestine specimens were deparaffinized, rehydrated in decreasing concentrations of ethanol, treated for 10 minutes with 3% H 2 O 2 to quench endogenous peroxidase activity and heated to 100C for 1 h in 10 mM sodium citrate buffer, pH 6, for antigen retrieval