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In contrast, WT cells exposed to methylene blue exhibited a slight decrease (at 1 h) in GSH and GS total levels, and a slight increase in GSSG, before returning to unstressed levels

FFA treatment elicited a more pronounced enhancement of the ascorbateglutathione cycle, where AsA content increased by 455.7% and GSH by 320.5% relative to CK-stressed controls, whereas GSSG levels declined by 49.3%, resulting in a 4.6-fold rise in the GSH/GSSG ratio (Fig