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the coupling agent used in the reaction can be selected from group consisting of [Ethylcyano (hydroxyimino)acetate-02)-tri-(l-pyrrolidinyl)-Phosphonium hexa fluorophosphate (PyOxim), ethyl-2 -cyano-2-(hydroxy amino) acetate (Oxyma pure), 0-(benzotriazol-l-yl)-N,N,N,N'- tetramethyluronium tetrafluoroborate (TBTU), diisopropyl carbodiimide (DIC), 1,3- dicyclohexylcabodiimide (DCC) , 0-(7 -azabenzotriazol- 1 -yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (HATU), 1 -(dimethyl aminopropyl)-3-ethylcarbodiimide hydrochloride (ED the reaction temperature may range from 25C to 30C

S3): DLin-KC2-DMA (S87825, Shanghai Yuan Ye Biotech, Shanghai, China), DSPC (S50960, Shanghai Yuan Ye Biotech, Shanghai, China), cholesterol (A10076, Shanghai Yuan Ye Biotech, Shanghai, China), and PEGDMG (S22698, Shanghai Yuan Ye Biotech, Shanghai, China) were dissolved in ethanol (R433208-1L, Aladdin, Shanghai, China)

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Homocysteine-induced apoptosis in endothelial cells coincides with nuclear NOX2 and peri-nuclear NOX4 activity