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Poor diet, pollution, stress, and timethey all chip away at it (Medical News Today)

Over time, the genome becomes increasingly dysregulated: DNA regions that should be silent become noisy, protective genes are switched off, and chromatin, the scaffold of DNA wrapped around histones (imagine them like packaging bundles), becomes condensed and brittle, losing its flexibility

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TNB undergoes a further ionization in neutral and alkaline pH in water to form the yellow-colored TNB 2 species, the absorbance of which is quantied using a plate reader at 412 nm