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After five minutes, initiate the reaction by adding 40 L of the NADPH Solution into each well (it is recommended to use a multi-channel pipette) and mix thoroughly

[6] [7] [10] GPx1 typically uses glutathione (GSH) as the reductant, but when glutathione synthetase (GSS) is, as in brain mitochondria, -glutamylcysteine can serve as the reductant instead

Ebbehj, Niels E., et al

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Research shows that men suffer from the disease up to four times more often than women, but with age this ratio decreases and after the age of 60