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Cyanine 7 was chosen for its excitation wavelength in the near-infrared region, allowing for deeper tissue penetration and minimal tissue autofluorescence, resulting in a lower background signal for emission measurements

doi: 10.1016/j.molmet.2021.101181 109 WillardFSDourosJDGabeMBShowalterADWainscottDBSuterTMet al

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Most clinical studies measured visible change between 4 and 12 weeks of consistent daily use, not days