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Cecal samples Cecal weight was measured since the weight of this portion of the intestine appears to be highly influenced by the microbiome (e.g., certain antibiotic treatments dramatically increase cecal weight 27,28,29,30,31 but not the colon 27 )

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[PubMed: 25932639] [PubMed: 36455010] Document what matters Lot number and COA reference Receipt date and initial storage condition Aliquoting date(s) and storage location Freeze-thaw count (if relevant) Any observed changes (color, clumping, unexpected assay drift) Practical do this first checklist Store lyophilized peptides at -20C or below in a dry, temperature-stable environment avoid frost-free freezers

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Purity and sterility testing protect against contaminated or degraded products that may trigger adverse reactions or produce no results