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Using mammalian cells in culture, we found that micromolar levels of homocysteine decreased GPx1 expression and activity without changing the levels of GPx1 mRNA [68]

Western blot and real-time PCR Real-time PCR was performed using a Roche LightCycler 480 detection system (Roche, Basel, Switzerland)

When MT2 reacted with AJO or ALL, the buffer was exchanged, effectively removing the dissociated Zn(II) from the sample

16 For example, bisphenols are a chemical family with well-studied toxic effects on the genome and epigenome (the structural aspect of genetic material which affects how genes are expressed)