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Sudden Worsening of Heart Failure: In rare cases, rapid correction of severe anaemia can increase blood volume and strain the heart

Next, the gels were equilibrated in transfer buffer (25 mM Tris base, 192 mM glycine, and 10% methanol) for 30 min at 4C, after which the proteins were transferred to polyvinylidene fluoride membranes (Bio-Rad Laboratories) at a constant current of 300 mA for 3 h at 4C

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Valente-Silva P, Ruas JL

This can lead to increased metabolism and reduced plasma concentrations of various antidepressants, potentially decreasing their efficacy