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There are at least two mechanisms by which estradiol increases the calcium transient consequent to depolarizing GABA: 1 ) an increase in the activity of NKCC1, the sodium-potassium-chloride cotransporter that pumps chloride into the cell and thereby maintains a chloride gradient conducive to depolarizing the membrane upon opening of the GABA A receptor, and 2 ) an increase in the 1c -subunit of the L-type voltage-gated calcium channel

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