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The reagent was added to the assay plate according to the product instruction and allowed to incubate on an orbital shaker for 3 min at room temperature before subjecting the plate to the GloMax luminometer (Promega)

Tutwiler V, Mukhitov AR, Peshkova AD, Le Minh G, Khismatullin RR, Vicksman J, et al

Tirzepatide does this by mimicking two hormones, GLP-1 and GIP, which help your pancreas release more insulin and reduce the amount of sugar your liver makes

Sci Rep (2021) 11:18522

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