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[DOI] [PubMed] [Google Scholar] 114.Esterbauer H., Schaur R.J., Zollner H

The gut microbiome and bone strength

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This was mixed vigorously for about 15 s and the homogenates were then kept at room temperature for 10 min followed by centrifugation at 12,000 rpm for 15 min at 4 C

Furthermore, each batch undergoes testing for endotoxins using the Limulus Amebocyte Lysate (LAL) assay to ensure it is free from bacterial contaminants that could interfere with experimental results, particularly in cell-based or in vivo studies