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Immunoblot assays Tissue samples were crushed on dry ice to obtain homogenous aliquots and then lysed in RIPA buffer (Thermo Scientific #89900) containing Halt protease & phosphatase inhibitor (Thermo Scientific #1861280)

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Researchers are expected to follow institutional biosafety rules, animal-care guidelines, and local regulations when working with KLOW, just as they would with any other unapproved laboratory reagent

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