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Estrogen deficiency and the decline of skin collagen were first observed in 1941 by Albright, who noticed that thin skin was more common in older women with osteoporotic fractures [21]

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However, ongoing research is needed to better understand how these findings translate to human health outcomes

However, to accurately assess the screening ability of these in vitro 3D bloodbrain barrier models for nanomedicines, it is first necessary to validate their barrier function