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and 4) modulation via peroxisome proliferator-activated receptor (PPAR) agonists of steatosis, inflammation, and fibrosis

Markers of protein oxidation (protein carbonyl glutamic [GSA] and aminoadipic [AASA] semialdehydes), glycoxidation (carboxyethyl-lysine [CEL], carboxymethyl-lysine [CML] and carboxymethyl-cysteine [CMC]), lipoxidation (malondialdehyde-lysine [MDAL]) and mitochondrial stress (2-succinyl-cysteine [2SC]) were identified as trifluoroacetic acid methyl ester derivatives in acid hydrolyzed delipidated and reduced plasma protein samples by GC/MS

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The slides were then blocked with 2% goat serum in 1 TBST for 1 h at room temperature, and incubated with anti-mouse PLIN2 antibody (Thermo Fisher Scientific Cat #15294-1AP), anti-mouse 4HNE antibody (Thermo Fisher Scientific Cat #MA5-27570) and anti-rabbit CYP2E1 antibody (Abcam Cat #ab28146)