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Beyond this classic signaling pathway that is widely implicated in tumorigenesis, GGCT could also regulate the Notch-Akt axis to facilitate the uncontrolled proliferation and aggressive growth of glioma cells [14]

At the indicated time points, 1 L of reaction was withdrawn, diluted 6-fold in Replication Stop Dye (3.6% SDS, 18 mM EDTA, 90 mM Tris-HCl pH 8, 90 mg/mL Ficoll, and 3.6 mg/mL Bromophenol Blue), incubated with 20 mg proteinase K at 21C for 16 h, and then resolved by 0.8% agarose gel electrophoresis

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