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The cells were then lysed to quantify the amounts of albumin inside, or washed and further incubated in serum-free medium for an additional 3 h to allow for release of recycled albumin back into the medium

These molecular alterations compromise cell integrity and cellular function, impairing cellular signalling, reducing growth factor responsiveness, and increasing cell death

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Moreover, it is characterized by a high prevalence of coexistence with many other diseases including metabolic disorders such as type 2 diabetes mellitus (T2DM) and obesity