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Generally, NNMT activity or expression is significantly elevated in cancers, such as brain [68], lung [69], liver [70], kidney [71, 72], bladder [73], stomach [74, 75], pancreas [76], colon [77], and oral [78, 79] cancer but cancer cells usually have a significantly higher anaerobic metabolism rate than normal cells

However, the high concentration makes it harder to measure very small doses accurately

The membranes were blocked with 5% skim milk in TBS/T (20 mM TrisHCl, pH 7.6, 137 mM NaCl and 0.05% Tween 20) and incubated with an anti-pAkt Ser 473 antibody (#9271