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After staining, plate was read on BMG CLARIOstar plate reader with FITC excitation/emission filter 485/528nm

The rationale is that under normal physiological circumstances, the decline in IGFBP-1 from its maximally stimulated overnight fasting level (34) to the level seen after food intake on a molar basis is relatively modest, and only leads to minor increases in serum free IGF-I (37, 38)