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Additionally, the research determined the semi-lethal concentration (LD50) and established the safe dosage range of CGA in Kunming (KM) mice through in vivo acute toxicity testing via tail vein injection

RNA-seq and analysis Total RNA isolation, cDNA library construction, and sequencing were performed at BGI (Beijing Genomics Institute, Shenzhen, Guangdong, China) using RNA-seq technology, as previously described 77,78

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Metabolic recycling of ammonia via glutamate dehydrogenase supports breast cancer biomass