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After harvesting, cells were plated to a density of 6 0.5 10 5 cells/mL in six-well plates (Corning) and allowed to incubate for 72 h at 37C in a 5% (v/v) CO 2 atmosphere to a confluence of 90%

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Approximately 4000 genetic diseases have been identified, and over 100 kinds of genetic diseases are newly discovered every year, posing serious threats to human health

120 In myeloid cell-specific SIRT1 knockout mice, lipid toxicity or LPS stimulation induced more severe hepatic inflammation, elevated pro-inflammatory cytokine levels, and enhanced M1 macrophage polarization, highlighting the critical role of SIRT1 in preserving immune balance