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At the end of the incubation period, medium was collected and centrifuged to remove any floating cells

Reconstituted material may be stored at 4 C for short-term use

Plus, it gets much easier after the first few times

The choice between 5-Amino-1MQ vs Tesofensine, or indeed any potent research compound, boils down to an intricate understanding of your specific hypotheses, the precise mechanisms you wish to investigate, and the unwavering demand for material purity

This is mechanistically attractive, because it would explain how a tetrapeptide can exert such diverse effects on pineal gland, retina and brain function