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Ni-NTA agarose resin (Qiagen, 30210) was equilibrated in buffer A (20 mM Tris-HCl pH 8.0 and 150 mM NaCl) and His-tagged laccases were bound to the resin

These effects of LA are in agreement with an alteration of the thiol reactivity of redox components of the insulin signaling pathway caused by a thiol/disulfide exchange mechanism

It was developed by Jackie Gartner-Schmidt and Amanda Gillespie

doi: 10.3389/fphar.2022.977385 Received 24 June 2022 Accepted 01 September 2022 Published 27 September 2022 Volume 13 - 2022 Edited by Sai Wang Seto, Hong Kong Polytechnic University, Hong Kong SAR, China Reviewed by Dominic Del Re, The State University of New Jersey, United States Karen R