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For spatial transcriptomics studies, the tongue tissues were rapidly harvested and flash frozen in liquid nitrogen

According to Ma and Farny (2023), senescence phenotypes in post-mitotic neurons cellular senescence are pronounced in enhanced activity of senescence-associated beta-galactosidase (SA--gal), cell cycle arrest [P53-P21 pathway and P16 pathway integrate to RB (retinoblastoma) hypophosphorylation], DNA damage, elevated RB hypophosphorylation, SASPs, and also senescence-associated heterochromatic foci (SAHFs), promoting cell cycle arrest (Ma and Farny, 2023

AuffenbergEHedrichUBSBarbieriRMielyDGroschupBWuttkeTVet al

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Fricker, L.B