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Data show that up to two-thirds of patients with HFpEF have CAD or coronary microvascular dysfunction including ischaemia, which leads to disruption of LV diastolic and systolic function, development of fibrosis, increased myocardial stiffness and cardiac remodelling

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Briefly, islets were handpicked, transferred to a petri dish containing 11 mL of KrebsRinger bicarbonate (KRB) buffer supplemented with 0.1% bovine serum albumin (BSA) and 3 mM glucose, and incubated at 37 C and 5% CO 2 for 60 min