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Despite all these knowledge, there are documented drawbacks to fish oil use, which includes the presence of heavy metal contamination and antibiotics in fish sources (Sidhu, 2003), as well as smells and odors that are undesirable, issues with stability, high cost, and challenges in purifying because of the small levels of DHA and EPA fatty acids ( Figure 1 3.1 Sources of long-chain polyunsaturated fatty acids 3.1.1 Microbial sources Microorganisms such as bacteria, fungi, algae, mosses, and protozoa can manufacture diverse polyunsaturated fatty acids (PUFAs) through aerobic and anaerobic pathways ( Schizochytrium sp , Yarrowia lipolytica , and Mortierella alpina , have been successfully utilized as primary sources of DHA, EPA, and ARA, respectively

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