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(2017) found in a mouse model of renal ischemia-reperfusion injury that the absence of ACSL4, a factor associated with ferroptosis, rendered cells more vulnerable to necroptosis over time

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The primary antibodies were diluted in Tx buffer (0.2% gelatin, 300 mM NaCl and 0.3% Triton X-100 in PBS) and incubated for 48 h at room temperature