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patent protection in September 2023, triggering an authorized generic entry deal and multiple biosimilar entrants

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Samples were incubated overnight with a primary antibody against -MSH (Bioss, MA, USA, # BS-1848R) or MC5-R (Alomone Labs, Jerusalem, Israel, # AMR-025) followed by biotinylated horseradish peroxidase-conjugated secondary antibody incubation and detection with diaminobenzidine (ABC kit, Vector Labs, Burlingame, USA)

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