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Acute respiratory allergy to HDM Female BALB/c By J mice (aged 7 weeks) were sensitized once per week for 4 weeks with 500 g HDM diluted in 20 l of 70% DMSO/PBS (phosphate-buffered saline) by the percutaneous route (Figure 1A)

Biochemical analyses revealed that compared to the Blank group, the Model group exhibited significantly elevated levels of GLU, FINS, HOMA-IR, LDL-C, TC, TG, TNF-, ALT, AST, and MDA, along with a marked decrease in HDL-C and the antioxidant markers SOD and GSH