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Quantitative PCR (qPCR) analysis of fractionated adipocytes in lean mice confirmed that NDI1 expression prevents the decline in Pparg , Ppargc1b and Bmper in mice lacking adipocyte Bmal1 (Extended Data Fig

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Cultures were then diluted 1:500 to 1:1,000 in TSB or Seahorse XF DMEM (Agilent) supplemented with 100 mM glucose, 2 mM L-glutamine and 1 mM sodium pyruvate, and dispensed into poly-D-lysine (PDL)-coated Seahorse XF HS miniplates (100 l per well)