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HMGB1 binds to its receptor, namely receptor for advanced glycation end products (RAGE) to trigger the downstream inflammatory response and produce various pro-inflammatory mediators, like tumour necrosis factor-alpha (TNF-) and interleukin-1beta (IL-1), leading to neuroinflammation and neuronal apoptosis (Lotze and Tracey 2005)

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Research-grade GLP-1 peptides require storage at -20C to -80C for long-term stability, reconstitution in appropriate buffers (pH 7.4-8.0), and protection from repeated freeze-thaw cycles to maintain biological activity

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That depth of evidence gives us substantially more confidence in its mechanisms and effects than we can have with newer, less-studied compounds