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For the reconstitution of egtA AfS77 , a functional copy of egtA (a 5691 bp PCR-amplified fragment generated with the primers oAetgA1.7_f and oAegtA1.8_r) was subcloned into pAN8.1 containing the phleomycin resistance cassette ( ble ) by a digestion with Sph I and Nhe I resulting in (p)egtA:ble

doi: 10.1016/j.molcel.2021.12.026 152 ShiJZhaoYWangKShiXWangYHuangHet al

doi: 10.3389/fendo.2023.1112363 56 SinghAKukretiRSasoLKukretiS