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After 30 min, dogs (5 per group) were administered with either: IntelliCap capsules (Medimetrics, Eindhoven, Netherlands) filled with 0.7 mL of peptide and permeation enhancer solution (J229 8 mg/mL, NaCDC 12 mg/mL)

The fluorescence was measured in an EnVision plate reader (PerkinElmer), and cAMP levels are expressed as a ratio of 665/620 nm

If youre new to glutathione, its wise to start at the lower end of this range to see how your body responds

Absorption of the His-tagged protein is performed at neutral to slightly alkaline pH to prevent protonation and loss of binding capacity of the weakly basic histidine imidazole groups