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Competition binding curves were performed in triplicate

Enzymatic activity was determined spectrophotometrically by measuring the change in absorbance at 460 nm over 3 min (Rittirsch et al., 2008)

We continue to believe that the loss of participation by ACOs unwilling to progress to two-sided risk with their second agreement period is balanced against, and outweighed by, the potential for increased effectiveness from other ACOs that continue to participate and successfully manage an earlier transition to performance-based risk, and establishing a policy that we believe will further advance the program's goals