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RNA extraction and qRTPCR analysis Total RNA was extracted by AG RNAex Pro RNA reagent (Agbio, Hunan) according to the manufacturers instructions, and reverse transcription was performed by premix reagent for qPCR (Agbio, Hunan)

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If Grx(SSR) intermediates and mixed disulfides with roGFP2 were more stable and were readily formed, glutaredoxins would sense and transfer a plethora of other redox states to roGFP2 probes

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