Had he even tried it
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After filtering the clarified cell lysate through a 0.45 m filter, the crude protein solution was loaded onto the column at a flow rate of 3 ml min 1
Cancer Cell 35:830849 Lu M, Zhou Y, Sun L, Shafi S, Ahmad N, Sun M et al (2022) The molecular mechanisms of ferroptosis and its role in glioma progression and treatment
ALA: Alpha-Linolenic Acid,GPX4: Glutathione Peroxidase 4, GSH: glutathione, CISD2: CDGSH, Iron Sulfur Domain 2, SLC7A11: Solute Carrier Family 7 Member 11, IGF2BP3: Insulin-Like Growth Factor 2 mRNA, Binding Protein 3, RM: Recurrent miscarriage, EMT: Epithelial-Mesenchymal Transition, HIF1: Hypoxia-Inducible Factor 1 Alpha, lnc-HZ06: Long Non-coding RNA HZ06, NCOA4: Nuclear Receptor Coactivator 4, lncRNA: Long Non-coding RNA, ROS: reactive oxygen species, TP53: Tumor Protein 53, EZH2: Enhancer of Zeste Homolog 2, TIMP1: Tissue inhibitor matrix metalloproteinase 1, SLC3A2: Solute Carrier Family 3 Member 2, GABARAPL2: Gamma-aminobutyric acid receptor-associated protein-like 2