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In addition, several penicillin-binding proteins ( BC1469, BC2190*, BC2448, BC2688, BC4075 ), which are involved in the final stages of PG synthesis, are upregulated in biofilms with a maximum expression level at 48 h, as well as three transpeptidases ( BC2468*, BC5027, BC0771 ), which catalyze the PG cross-linking (Fig

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